Wet Lab Offerings…

 

1. Nucleic Acid Extraction
2. NGS library preparation
3. DNA sequencing on Illumina Platform
   • Whole Genome Sequencing
   • Whole Exome Sequencing
   • mRNA Sequencing
   • Total RNA Sequencing
   • Targeted DNA Panel Sequencing
   • Targeted RNA Panel Sequencing
   • Targeted Gene-Fusion
   • miRNA Sequencing
   • ChIP DNA Sequencing
   • DeNovo Sequencing
   • 16s-MetaGenomics Sequencing
   • Shotgun MetaGenomics
   • Methylome Sequencing
   • Targeted Methylome Sequencing
   • PCR-Amplicon Sequencing
   • Custom-DNA/RNA/Gene-fusion Panel Sequencing

Dry Lab Offering…

1. FastQ Data QC
2. FastQ Data trimming
3. Whole Genome Sequencing (WGS):
   1. Alignment and mapping
   2. Variant calling
   3. CNV identification
4. mRNA/totalRNA Sequencing:
   1. Transcriptome Mapping
   2. Differential Expression
   3. Volcano Plots
   4. PCA-Analysis
   5. Venn-Diagram
   6. HeatMap
   7. Enrichment-Analysis:
      1. Molecular Function
      2. Biological Processes
      3. Disease
5. Targeted DNA Panel Sequencing:
   1. Alignment & Mapping with target region
   2. Variant calling
   3. Variant annotation
   4. Variant Interpretation
   5. CNV Analysis
6. Targeted RNA Panel Sequencing:
   1. Alignment & Mapping with target region
   2. Differential expression
   3. Panel specific RNA expression and fusion calling
   4. PCA-Analysis
   5. Volcano Plot
   6. Venn-diagram
   7. HeatMap
7. Targeted Gene-Fusion:
   1. Alignment and Mapping with target region
   2. Gene fusion detection
8. miRNA Sequencing:
   1. Alignment and mapping with miRNA database
   2. Differential expression
   3. Volcano plot
   4. PCA-Analysis
   5. HeatMap
9. ChIP DNA Sequencing:
   1. Mapping with Genome
   2. Peak calling
   3. Annotation
   4. Biological Interpretation
10. DeNovo Sequencing:
    1. Long-range denovo sequencing
    2. Short-range denovo sequencing
    3. Hybrid denovo sequencing
    4. Contig > Scaffold > Chromosome assembly
    5. Genome Gap-filling
    6. Genome annotation
    7. Novel pathogen identification
11. 16s MetaGenomics Sequencing:
    1. Data QC
    2. OTU Clustering (Genus and Species level)
    3. Alpha and Beta diversity
12. MetaTrancriptome Sequencing:
    1. Find prokaryotic gene
    2. Build functional profile
    3. Functional abundance table
    4. Drug Resistance analysis
13. Shotgun Metagenomics
    1. Denovo Metagenome
    2. Differential Abundance analysis
    3. HeatMap of abundance table
14. Methylome Sequencing:
    1. Map complete DNA CpG methylomes
    2. Bisulfite-seq methylation site detection
    3. Compare CpG Methylome
    4. Predict Methylation profile
    5. Phenotypic effect prediction
    6. Targeted Methylome sequencing analysis
15. PCR-Amplicon Sequencing
    1. Long Range PCR Analysis
    2. SNP detection
    3. Methylome analysis
    4. 16s-Metagenomics
    5. Targeted Panel analysis

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